spectral separation of quantum dots within tissue equivalent phantom using linear unmixing methods in multispectral fluorescence reflectance imaging

Spectral Separation of Quantum Dots within Tissue Equivalent Phantom Using Linear Unmixing Methods in Multispectral Fluorescence Reflectance Imaging

Introduction Non-invasive Fluorescent Reflectance Imaging (FRI) is used for accessing physiological and molecular processes in biological media. The aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using SVD, Jacobi SVD, and NMF methods in the FRI mode. Materials and Methods In this article, a tissue-like phantom and an optical...

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom

development of different optical methods for determination of glucose using cadmium telluride quantum dots and silver nanoparticles

a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...

Geometrical Endmember Extraction and Linear Spectral Unmixing of Multispectral Image

Accurate mapping is prepared using Linear unmixing of satellite images. Endmember extraction contributes the unmixing accuracy. In this paper, Endmembers are extracted using different Geometrical algorithms like Pixel Purity Index (PPI), Nearest Finder (N-FINDR) and Sequential Maximum Angle Convex Cone (SMACC) algorithms. Extracted Endmembers are given as input for unmixing and it is attempted ...

Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin

Understanding the skin penetration of nanoparticles (NPs) is an important concern due to the increasing presence of NPs in consumer products, including cosmetics. Technical challenges have slowed progress in evaluating skin barrier and NP factors that contribute to skin penetration risk. To limit sampling error and other problems associated with histological processing, many researchers are imp...

Noninvasive vascular imaging in fluorescent tumors using multispectral unmixing.

Noninvasive imaging of tumor vascularization in animal models provides an important tool for studying the biology of tumor angiogenesis as well as monitoring the effects of antiangiogenic therapies. Through the use of in vivo multispectral fluorescent imaging, we have discovered a distinct spectral signature associated with blood vessels present in fluorescent tumors in mice. This unique spectr...